Catechin gallates are NADP+-competitive inhibitors of glucose-6-phosphate dehydrogenase and other enzymes that employ NADP+ as a coenzyme

Eui Seok Shin; Jiyoung Park; Jae-Min Shin; Dooho Cho; Si Young Cho; Dong Wook Shin; Mira Ham; Jae Bum Kim; Tae Ryong Lee

doi:10.1016/j.bmc.2008.02.030

Catechin gallates are NADP+-competitive inhibitors of glucose-6-phosphate dehydrogenase and other enzymes that employ NADP+ as a coenzyme

Bioorg Med Chem. 2008 Apr 1;16(7):3580-6. doi: 10.1016/j.bmc.2008.02.030. Epub 2008 Feb 14.

Authors

Eui Seok Shin¹, Jiyoung Park, Jae-Min Shin, Dooho Cho, Si Young Cho, Dong Wook Shin, Mira Ham, Jae Bum Kim, Tae Ryong Lee

Affiliation

¹ R&D Center, AmorePacific Corporation, 314-1 Bora-Dong, Giheung-Gu, Yongin, Gyeonggi 446-729, Republic of Korea.

PMID: 18313308
DOI: 10.1016/j.bmc.2008.02.030

Abstract

Recent studies have shown that glucose-6-phosphate dehydrogenase (G6PD) is an effectual therapeutic target for metabolic disorders, including obesity and diabetes. In this study, we used in silico and conventional screening approaches to identify putative inhibitors of G6PD and found that gallated catechins (EGCG, GCG, ECG, CG), but not ungallated catechins (ECG, GC, EC, C), were NADP(+)-competitive inhibitors of G6PD and other enzymes that employ NADP(+) as a coenzyme, such as IDH and 6PGD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3-L1 Cells
Adipocytes / drug effects
Adipocytes / enzymology
Animals
Catechin / analogs & derivatives*
Catechin / chemistry
Catechin / pharmacology
Coenzymes / metabolism*
Glucosephosphate Dehydrogenase / antagonists & inhibitors*
Glucosephosphate Dehydrogenase / metabolism*
Humans
Kinetics
Mice
Molecular Structure
NADP / metabolism*
Structure-Activity Relationship

Substances

Coenzymes
NADP
catechin gallate
Catechin
Glucosephosphate Dehydrogenase